shrna primer
To alleviate these problems, the long reverse primer can be exchanged for two shorter primers. The reaction is then modified to a two-round ...,To express your shRNA of interest using the pENTR™/H1/TO vector, simply: Clone your ds ... Primer. Sequence. Amount. H1 Forward. 5'-tgttctgggaaatcaccata-3'.
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 shrna primer 相關參考資料
Design and Cloning of an shRNA into a Lentiviral Silencing ...
The design uses a 5′ forward primer upstream of the pol III promoter and a 3′ reverse primer that includes the entire shRNA sequence (i.e., sense, loop, and ... http://cshprotocols.cshlp.org Design and cloning strategies for constructing shRNA ... - NCBI
To alleviate these problems, the long reverse primer can be exchanged for two shorter primers. The reaction is then modified to a two-round ... https://www.ncbi.nlm.nih.gov Inducible shRNA Expression Vectors - Thermo Fisher Scientific
To express your shRNA of interest using the pENTR™/H1/TO vector, simply: Clone your ds ... Primer. Sequence. Amount. H1 Forward. 5'-tgttctgggaaatcaccata-3'. https://www.thermofisher.com Plasmid DNA - 中研院RNAiCore 核心設施
Lentivirus-based shRNA表達轉移質體(pLKO_AS1系列質體)。請見質體資訊詳細 ... Sequencing Primer，請參閱(Protocol : Sequencing Primer). 4. Purification of ... http://rnai.genmed.sinica.edu. Protocol for shRNA construction: PCR method
shRNA oligonucletide (50μM). 1μl. Forward primer (100μM). 2μl. Reverse primer (100μM). 2μl. KAPAHiFi DNA Polymerase. 1μl. 5X GC buffer. 10μl. 2mM dNTPs. http://rnai.genmed.sinica.edu. RNA Interference - 第 151 頁 - Google 圖書結果
The most outstanding feature of this PCR reaction is the length of the reverse primer that encodes the shRNA transcription template (Fig. 2B). Again, the shRNA ... https://books.google.com.tw Short Hairpin (shRNA) Cloning Vectors | OriGene
shRNA mediated gene knockdown is still a popular gene function study tool. OriGene has 10 shRNA cloning vectors, including retroviral, lentiviral and AAV ... https://www.origene.com Short hairpin RNA (shRNA) - Horizon Discovery
microRNA-adapted shRNAs. SMARTvector lentiviral shRNA. Guaranteed gene silencing with state-of-the-art shRNA designs and multiple promoter and reporter ... https://horizondiscovery.com Short Hairpin RNA (shRNA): Design, Delivery, and ...
Design two sequencing primers, one for either strand of the vector into which the shRNA is cloned. Primers should lie approximately 50–100 bp upstream (for ... https://www.ncbi.nlm.nih.gov 技術支援 - 中研院RNAiCore 核心設施
註一: pLKO.1-puro轉移載體以U6 promoter表達shRNA，本核心設施使用下之primers定序之： (1) LKO_shRNA/F (forward primer)： 5'-acaaaatacgtgacgtag-3' (for ... http://rnai.genmed.sinica.edu. |