sgrna wiki
Scientists can use viral or non-viral systems for delivery of the Cas9 and sgRNA into target cells. Electroporation of DNA, RNA or ribonucleocomplexes is the most common and cheapest system. This technique was used to edit CXCR4 and PD-1, knocking in new ,CRISPR interference (CRISPRi) is a genetic perturbation technique that allows for sequence-specific repression or activation of gene expression in prokaryotic and eukaryotic cells. It was first developed in the Lim and Weissman labs at UCSF. Based on the
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Cas9 - Wikipedia
跳到 The interactions between sgRNA and Cas9 - Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR adaptive immunity system in Streptococcus pyogenes,... https://en.wikipedia.org CRISPR - Wikipedia
Scientists can use viral or non-viral systems for delivery of the Cas9 and sgRNA into target cells. Electroporation of DNA, RNA or ribonucleocomplexes is the most common and cheapest system. This tech... https://en.wikipedia.org CRISPR interference - Wikipedia
CRISPR interference (CRISPRi) is a genetic perturbation technique that allows for sequence-specific repression or activation of gene expression in prokaryotic and eukaryotic cells. It was first develo... https://en.wikipedia.org CRISPR-Display - Wikipedia
CRISPR-Display (CRISP-Disp) is a modification of the CRISPR/Cas9 system for genome editing. The CRISPR/Cas9 system uses a short guide RNA (sgRNA) sequence to direct a Streptococcus pyogenes Cas9 nucle... https://en.wikipedia.org CRISPRCas Tools - Wikipedia
This page describes a list of software platforms and bioinformatics tools built to facilitate the design of guide RNAs (gRNAs) for use with the CRISPR/Cas system. The CRISPR/Cas system was originally ... https://en.wikipedia.org CRISPRCas-Methode – Wikipedia
Die CRISPR/Cas-Methode (Clustered Regularly Interspaced Short Palindromic Repeats) ist eine biochemische Methode, um DNA gezielt zu schneiden und zu verändern (Genome Editing). Gene können mit dem CRI... https://de.wikipedia.org CRISPRCpf1 - Wikipedia
Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella 1 or CRISPR/Cpf1 is a DNA-editing technology analogous to the CRISPR/Cas9 system. Cpf1 is an RNA-guided endonu... https://en.wikipedia.org Protospacer adjacent motif - Wikipedia
Protospacer adjacent motif (PAM) is a 2-6 base pair DNA sequence immediately following the DNA sequence targeted by the Cas9 nuclease in the CRISPR bacterial adaptive immune system. PAM is a component... https://en.wikipedia.org sgRNA Selection - Clontech
Choosing a Target Sequence for CRISPR/Cas9 Gene Editing. CRISPR/Cas9 gene targeting requires a custom single guide RNA (sgRNA) that contains a targeting sequence (crRNA sequence) and a Cas9 nuclease-r... http://www.clontech.com 引导核糖核酸- 维基百科，自由的百科全书
嚮導RNA（guide RNA，gRNA），也稱為小嚮導RNA（small guide RNA，sgRNA）。是作用於動質體（kinetoplastid）體內一種稱為RNA編輯（RNA editing）的後轉錄修飾過程中。也是一種小型非編碼RNA。可與pre-mRNA配對，並在其中插入一些尿嘧啶（U），產生具有作用的mRNA。 https://zh.wikipedia.org |