sgRNA protocol
CRISPR/Cas9 gene targeting requires a custom single guide RNA (sgRNA) that contains a targeting sequence (crRNA sequence) and a Cas9 ... ,Separate protocols are available for the entire genome-scale CRISPR knock-out (GeCKO) library. This protocol is for creating individual lentiCRISPR targeting a ...
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 sgRNA protocol 相關參考資料
C6-15產品暨服務簡介 - 中研院RNAiCore 核心設施
Two vector system: sgRNA expression vectors (C6-8-64 ~ C6-8-66) 此系列質 ... sgRNA construction(Annealing method) (Protocol : sgRNA cloning Protocol V1). http://rnai.genmed.sinica.edu. How to design sgRNA sequences - Takara Bio
CRISPR/Cas9 gene targeting requires a custom single guide RNA (sgRNA) that contains a targeting sequence (crRNA sequence) and a Cas9 ... https://www.takarabio.com LentiCRISPR lentiviral CRISPRCas9 and single ... - Addgene
Separate protocols are available for the entire genome-scale CRISPR knock-out (GeCKO) library. This protocol is for creating individual lentiCRISPR targeting a ... https://media.addgene.org protocol for cloning sgRNA - Addgene
sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested with SapI creating sticky ends for ligation of annealed and phosphorylated ... https://media.addgene.org Protocol for one sgRNA cloning Simplified protocol - Addgene
Protocol for one sgRNA cloning. Simplified protocol. 1. Manually search for 23-bp target sites (5'-N20NGG-3') within exons of genomic DNA sequences of genes ... https://media.addgene.org Protocol for sgRNA construction (Annealing method)
Prepare sgRNA cloning vector: Protocol for sgRNA construction (Annealing method). Digest 5~10 μg of sgRNA expressing vector (pU6-gRNA.Ppuro) with BsmBI ... http://rnai.genmed.sinica.edu. Protocol: Cloning of oligos for sgRNA (CRISPR) or shRNA ...
This protocol allows you to clone oligos to generate shRNAs or sgRNAs on a small scale. -For sgRNAs, pXPR vectors with a single BsmBI or BbsI cloning site are ... https://portals.broadinstitute Protocols for CRISPR sgRNA (aRGENs)
Protocols for CRISPR sgRNA (aRGENs). 1. Reconstitution of lyophilized RNA (single guide RNA ,. sgRNA). - Dissolve lyophilized guide RNA in nuclease-free ... http://www.toolgen.com sgRNA Quick Start Guide - Synthego
Simplify Analysis. Learn about our ICE tool for analyzing editing efficiency. Download Protocol ... https://www.synthego.com Zhang Lab CRISPR Page - Addgene
CRISPR Cas9 gRNA plasmids from the Zhang lab. ... as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols 2013). https://www.addgene.org |