delta 8.91 vector

Type, Name, Description, Start Position, End Position. promoter, CMV, 73, 599. viral gene, Gag-Pol, packaging plasmid, 6...

delta 8.91 vector

Type, Name, Description, Start Position, End Position. promoter, CMV, 73, 599. viral gene, Gag-Pol, packaging plasmid, 600, 5734. polypurine tract, cppt, 4850 ... ,Ratio of 4:3:1 of transfer vector (tet-pLKO-puro or pCW57.1), pCMV delta 8.91 viral gene plasmid, pMD2.G VSV-G envelope plasmid. 3 µg PEI is used for every 1 ...

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delta 8.91 vector 相關參考資料
Description:

Multiply attenuated lentiviral vector keeps the ability to transduce growth-arrested cells and monocyte-derived macrophages in culture, and could also deliver ...

http://rnai.genmed.sinica.edu.

dR8.91 - Vector Detail

Type, Name, Description, Start Position, End Position. promoter, CMV, 73, 599. viral gene, Gag-Pol, packaging plasmid, 600, 5734. polypurine tract, cppt, 4850 ...

https://plasmid.med.harvard.ed

Help increasing in vitro infection with lentivirus : labrats - Reddit

Ratio of 4:3:1 of transfer vector (tet-pLKO-puro or pCW57.1), pCMV delta 8.91 viral gene plasmid, pMD2.G VSV-G envelope plasmid. 3 µg PEI is used for every 1 ...

https://www.reddit.com

Note: Lentivirus Vector Gene Expression during ES Cell ...

G encoding VSV-G, 10 μg of packaging plasmid pCMVΔR 8.91 (37) expressing Gag, Pol, Tat, and Rev, and 20 μg of vector plasmid HIV-PGK-GFP, which ...

https://www.ncbi.nlm.nih.gov

pCMV delta R8.2 - Addgene

Plasmid pCMV delta R8.2 from Dr. Didier Trono's lab contains the insert HIV-1 GAG/POL, Tat and Rev. This plasmid is available ... Vector backbone. pCMVR8.2.

https://www.addgene.org

Plasmid DNA - 中研院RNAiCore 核心設施

此系列質體皆為bicistronic mRNA expression vector。第一個蛋白由CMV啟動子轉錄之cap-dependent mRNA轉譯;第二個蛋白則以EMCV的IRES(cap- ...

http://rnai.genmed.sinica.edu.

Vector Database - pCMV-dR8.91 - Addgene

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the ...

https://www.addgene.org

Vector資訊 - 中研院RNAiCore 核心設施

2. pCMVΔR8.91. * 以上Lentiviral transfer vector本核心皆有提供,如需申請, ...

http://rnai.genmed.sinica.edu.

viral vector icon Lentiviral Plasmids - Addgene

12263, pCMV delta R8.2, 2nd, Packaging, Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation transfer plasmids, Trono. 12259, pMD2.

https://www.addgene.org

What is the optimal amount of each lentiviral vector (2nd and ...

2nd gen: 10ug packaging (delta 8.2), 10ug transfer plasmid (pSPAX2), 5ug ... (lentiviral backbone vector), 0.5µg of pMD2-VSVG and 1.1µg of pCMV-R8.91 ...

https://www.researchgate.net