de3 lysogen

The strain carries both the DE3 lysogen and the plasmid pLysS. pLysS constitutively expresses low levels of T7 lysozyme,...

de3 lysogen

The strain carries both the DE3 lysogen and the plasmid pLysS. pLysS constitutively expresses low levels of T7 lysozyme, which reduce basal expression of ... , lambda DE3 is a phage construct that expresses T7 RNA polymerase under the control of a lacUV5 promoter. Studier and Moffat constructed ...

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de3 lysogen 相關參考資料
A Practical Hand Book of Genes to Clones -Manual - Google 圖書結果

Once a DE3 lysogen is formed, the only promoter known to direct transcription of the T7 RNA polymerase gene is the lacUV5 promoter, which is inducible by ...

https://books.google.com.tw

Bacterial Strains for Protein Expression

The strain carries both the DE3 lysogen and the plasmid pLysS. pLysS constitutively expresses low levels of T7 lysozyme, which reduce basal expression of ...

https://www.promega.com

lambda DE3 - EcoliWiki

lambda DE3 is a phage construct that expresses T7 RNA polymerase under the control of a lacUV5 promoter. Studier and Moffat constructed ...

https://ecoliwiki.org

Lysogen - Wikipedia

A lysogen or lysogenic bacterium is a bacterial cell which can produce and transfer the ability to produce a phage. A prophage is either integrated into the host ...

https://en.wikipedia.org

Producing Biomolecular Substances with Fermenters, ...

TABLE 4.1 Construction of Host Strain for Production Author: Earl Jones Date: November 4, 1989 Notes: Protocol for construction of DE3 lysogen; origin of ...

https://books.google.com.tw

RNA Interference - 第 44 頁 - Google 圖書結果

The genotype of E. coli HT115 is FÀ, mcrA, mcrB, IN(rrnD-rrnE)1, lambda-, rnc14::Tn10 (DE3 lysogen: lacUV5 promoter-T7 polymerase). T7 polymerase gene ...

https://books.google.com.tw

What is the difference between BL21 and BL21(DE3 ... - NEB

The DE3 designation means that respective strains contain the λDE3 lysogen that carries the gene for T7 RNA polymerase under control of the ...

https://www.neb.com

λDE3 Lysogenization Kit - Novagen | 69734 - Merck Millipore

200 µl, Helper Phage Lysate. •, 200 µl, Selection Phage Lysate. •, 50 µl, T7 Tester Phage Lysate. •, 200 µl, HMS174(DE3) glycerol stock. Quality Level, MQ100 ...

https://www.merckmillipore.com