Abcam IHC protocol
Shorter protocol (lengthy fixation step usually not required). Used with thicker sections (>25 µm): allows greater analysis of the 3D structure of the tissue. ,Step-by-step protocols for automated IHC with Anti-PD-L1 [clone 28-8] using Biogenex i6000, LEICA BOND RX, Ventana Ultra, and Dako Omnis.
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 Abcam IHC protocol 相關參考資料
IHC-PARAFFIN PROTOCOL (IHC-P)
Immunohistochemistry (or IHC) is a method for demonstrating the presence and location of proteins in tissue sections. Though less sensitive quantitatively ... https://www.abcam.com Immunohistochemistry (IHC): the complete guide
Shorter protocol (lengthy fixation step usually not required). Used with thicker sections (>25 µm): allows greater analysis of the 3D structure of the tissue. https://www.abcam.com Protocols for Immunohistochemistry (IHC)
Step-by-step protocols for automated IHC with Anti-PD-L1 [clone 28-8] using Biogenex i6000, LEICA BOND RX, Ventana Ultra, and Dako Omnis. https://www.abcam.com IHC protocol: paraffin, frozen and free floating sections
Paraffin and frozen sections · Apply fluorophore-conjugated secondary antibody to the slide diluted in TBS with 1% BSA. · Incubate for 1 h at room temperature. https://www.abcam.com IHC antigen retrieval protocol
Learn how to perform heat-induced or enzymatic antigen retrieval for IHC with our detailed IHC antigen retrieval protocol. https://www.abcam.com IHC training | Abcam
An IHC stain relies on antibodies that recognize the target antigen. You can use either chromogenic or fluorescent-based detection systems to visualize this ... https://go.myabcam.com IHC staining protocol
Most antibodies will be used in IHC-P at a concentration of 0.5–10 μg/mL. The primary antibody should be raised in a species different from the tissue being. https://docs.abcam.com IHC for brain slice sections video protocol
Learn how to visualize your proteins of interest with IHC on brain slice sections after electrophysiology recording (4:35 minutes). · Tissue fixation · Day 1. https://www.abcam.co.jp IHC for paraffin embedded sections video protocol
https://www.youtube.com IHC training Part 2: Immunostaining
In Part 2 of our IHC training, you'll learn how to stain your tissues. We'll run through the IHC protocol steps, such as antigen retrieval, permeabilization, ... https://go.myabcam.com |